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The objective of this slide presentation was to evaluate enumeration methods for the detection ofcoliform bacteria in water samples using a precise numberof organisms. Escherichia coli (ATCC = 11775) and Enterobacteraerogenes (ATCC = 13048) BioBall cultures were used.All methods were tested 10 times using each culture andthe mean number ¿¿¿¿ SD of all readings was calculated. Eight methods were evaluated using BioBall cultures:membrane filtration;mFC and mLS;defined substrate technology systems;Colilert and Colisuremultiple tube fermentation (MPN);Petrifilm;standard plate count; and, pour and spread plate method. Results of the methods are given, and conclusions were that: Petrifilm and PCA-P produced the most accurate andconsistent results(1 mL);Colilert produced the most consistent results with the lowestcoefficient of variation (100 mL);MF, Colisure and MPN may be producing results withgreater variability; and,BioBall is a very useful quality control tool for evaluatingthe efficiency of water testing procedures. Includes figures.

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Edition: Vol. - No. Published: 06/01/2006 Number of Pages: 38File Size: 1 file , 1 MB