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Conventional culture methods for the detection of Legionella species often requirefiltration and centrifugation to concentrate target organisms and subsequentdecontamination prior plating on highly selective agar media. Aliquots of sampleconcentrates may subsequently be treated by heat or acid to reduce or eliminate highlevels of non-target organisms that may be present in the sample. These treatmentshave been shown to reduce the total viable Legionella counts (TVLC).Immunomagnetic separation, utilizing beads coated with antibodies to a specifictarget organism, has been shown to be an effective strategy for isolating targetorganisms from complex environmental, food and clinical samples (Riffard et al.,2001; Yanez et al., 2005). This approach may eliminate the need for sample pretreatmentsand reduces the need for highly selective culture medium.The purpose of this evaluation was to compare the performance of Dynabeads anti-Legionella using and an automated immunomagnetic separation protocol using theBead Retreive with a standard protocol for the isolation and quantification ofLegionella pneumophila in environmental samples. Includes tables.

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Edition: Vol. - No. Published: 11/01/2005 Number of Pages: 4File Size: 1 file , 160 KB